Objective To investigate the effect of growth factor combined with autologous fat transplantation in facial rejuvenation. Methods A total of 100 patients with facial rejuvenation treated in our hospital from June 2019 to June 2021 were selected and divided into control group and study group according to different facial rejuvenation treatment methods, with 50 cases in each group. The control group was only treated with autologous fat transplantation, and the study group was treated with growth factor combined with autologous fat transplantation. The wrinkle improvement, treatment satisfaction, complications and secondary operation rate were compared between the two groups after treatment. Results The score of facial wrinkle scale in the study group was lower than that in the control group, and the difference was statistically significant (P＜0.05). The satisfaction of patients in the study group was 96.00%, which was higher than 70.00% in the control group, and the difference was statistically significant (P＜0.05). The incidence of complications in the study group was 4.00%, which was lower than 32.00% in the control group, and the difference was statistically significant (P＜0.05). The secondary operation rate of the study group was 4.00%, which was lower than 22.00% of the control group, and the difference was statistically significant (P＜0.05). Conclusion For patients needing facial rejuvenation treatment, growth factor combined with autologous fat transplantation can improve the wrinkle condition of patients, reduce the incidence of complications and secondary operation rate, which has high application value.