Objective To study the clinical effect of nano-microneedle into lyophilized powder in the treatment of sensitive skin. Methods A total of 60 patients with sensitive muscle treated in our hospital from January 2019 to January 2020 were randomly divided into observation group and control group, with 30 cases in each group. The control group was treated with conventional symptomatic treatment, and the observation group was treated with nano-microneedle into lyophilized powder on the basis of the control group. The clinical efficacy, non-invasive physiological indexes of facial skin (water content, oil content, pH level) and adverse reactions before and after treatment were compared between the two groups. Results The total effective rate of the observation group was higher than that of the control group (P＜0.05). The skin water content and oil content of the two groups were higher than those before treatment, and the pH was lower than that before treatment, meanwhile the water content and oil content of the observation group were higher than those of the control group, and the pH was lower than that of the control group (P＜0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P＞0.05). Conclusion Nanomicroneedle into lyophilized powder is safe and effective in the treatment of sensitive skin, which can improve skin water content and oil content. The curative effect of cosmetic skin is accurate and worthy of application.