罗方军(1971.8-),男,四川乐山人,本科,副主任医师,主要从事真菌性皮肤病及损容性皮肤病的诊治研究
R622
广州市科技计划项目（编号：201902010003）
广州市科技计划项目（编号：201902010003）
目的 探索新型材料β-葡聚糖联合低交联透明质酸钠在机体内的安全性以及对新生血管及胶原生成的影响。方法 将SPF级Wistar大鼠30只随机分为低交联透明质酸钠组、交联透明质酸钠（大分子）组、β-葡聚糖组、β-葡聚糖＋低交联透明质酸钠组、交联透明质酸钠（大分子）＋低交联透明质酸钠组，分别给予注射用低交联透明质酸钠1 ml、注射用交联透明质酸钠（大分子）1 ml、β-葡聚糖1 ml、β-葡聚糖+注射用低交联透明质酸钠1 ml、注射用交联透明质酸钠（大分子）+注射用低交联透明质酸钠1 ml。于第7、14及28天观察小鼠皮下组织，分析各组苏木精-伊红染色结果、胶原纤维含量面积、毛细血管及小血管数量。结果 注射β-葡聚糖联合低交联透明质酸钠的区域相较于其他注射材料其组织相容性更优，胶原纤维及血管新生更显著，并且注射部位未见肌肉肿胀和不良反应的发生。各组大鼠切片图像胶原纤维含量比较，差异有统计学意义（P＜0.05）；其中β-葡聚糖＋低交联透明质酸钠组胶原纤维含量最高，其次是交联透明质酸钠＋低交联透明质酸钠组，低交联透明质酸钠组、交联透明质酸钠组和β-葡聚糖组胶原纤维含量低于前两组。交联透明质酸钠组和β-葡聚糖＋低交联透明质酸钠组的毛细血管及小血管数量高于低交联透明质酸钠组、β-葡聚糖组和交联透明质酸钠＋低交联透明质酸钠组，差异有统计学意义（P＜0.05）；交联透明质酸钠组与β-葡聚糖＋低交联透明质酸钠组的毛细血管及小血管数量比较，差异无统计学意义（P＞0.05）；低交联透明质酸钠组与β-葡聚糖组和交联透明质酸钠＋低交联透明质酸钠组的毛细血管及小血管数量比较，差异无统计学意义（P＞0.05）。结论 β-葡聚糖联合低交联透明质酸钠皮下注射具有良好的组织相容性，且其胶原纤维含量面积、毛细血管及小血管数量优于其他注射材料，对新生血管生成及新生胶原具有明显且较安全的作用。
Objective To explore the safety of a new material β-glucan combined with low cross-linked sodium hyaluronate in vivo and its effecton angiogenesis and collagen production. Methods Thirty SPF Wistar rats were randomly divided into low cross-linked sodium hyaluronate group, cross-linked sodium hyaluronate (macromolecule) group, β-glucan group, and β-glucan+low cross-linked hyaluronate sodium group, cross-linked sodium hyaluronate (macromolecule)+low cross-linked sodium hyaluronate group, respectively, low cross-linked sodium hyaluronate for injection 1 ml, cross-linked sodium hyaluronate for injection (macromolecule) 1 ml, β-glucan 1 ml, β-glucan+low cross-linked sodium hyaluronate for injection 1 ml, cross-linked sodium hyaluronate for injection (macromolecule)+low cross-linked sodium hyaluronate for injection 1 ml. The subcutaneous tissue of mice was observed on the 7th, 14th and 28th days, and the results of hematoxylin-eosin staining, the collagen fibercontent and the number of capillaries and small blood vessels were analyzed in each group. Results Compared with other injection materials, the area injected with β-glucan combined with low cross-linked sodium hyaluronate had better histocompatibility, more significan collagen fiber and angiogenesis, and no muscle swelling and no adverse reactions at the injection site. There was significant differencein the collagen fibercontent in the slice images of rats in each group (P<0.05), among them, the collagen fibercontent in the β-glucan+low-crosslinking sodium hyaluronate group was the highest, followed by the cross-linking sodium hyaluronate+low-crosslinking sodium hyaluronate group, and the collagen fibercontent in the low-crosslinking sodium hyaluronate group, the cross-linking sodium hyaluronate group and the β-glucan group was lower than that in the firsttwo groups. The number of capillaries and small blood vessels in the cross-linked sodium hyaluronate group and the β-glucan+low cross-linked sodium hyaluronate group was higher than that in the low cross-linked sodium hyaluronate group, the β-glucan group and the cross-linked transparent sodium hyaluronate+low cross-linked sodium hyaluronate group, the differencewas statistically significant(P<0.05); there was no significantdifferece in the number of capillaries and small blood vessels between the cross-linked sodium hyaluronate group and the β-glucan+low cross-linked sodium hyaluronate group (P>0.05); there was no significantdifferencein the number of capillaries and small blood vessels between the low cross-linked sodium hyaluronate group and the β-glucan group and the cross-linked sodium hyaluronate+low cross-linked sodium hyaluronate group (P>0.05). Conclusion The subcutaneous injection of β-glucan combined with low cross-linked sodium hyaluronate has good histocompatibility. By comparing the area of collagen fibers,the number of capillaries and small blood vessels of differentcombination injection materials, it is better than other injection materials. Angiogenesis and new collagen have obvious and safe effects
方明星,吴伟东,王 瑛,等.β-葡聚糖联合低交联透明质酸钠在机体内应用的再生评价[J].医学美学美容,2022,31(2):1-7.
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