Objective To explore the safety of a new material β-glucan combined with low cross-linked sodium hyaluronate in vivo and its effecton angiogenesis and collagen production. Methods Thirty SPF Wistar rats were randomly divided into low cross-linked sodium hyaluronate group, cross-linked sodium hyaluronate (macromolecule) group, β-glucan group, and β-glucan+low cross-linked hyaluronate sodium group, cross-linked sodium hyaluronate (macromolecule)+low cross-linked sodium hyaluronate group, respectively, low cross-linked sodium hyaluronate for injection 1 ml, cross-linked sodium hyaluronate for injection (macromolecule) 1 ml, β-glucan 1 ml, β-glucan+low cross-linked sodium hyaluronate for injection 1 ml, cross-linked sodium hyaluronate for injection (macromolecule)+low cross-linked sodium hyaluronate for injection 1 ml. The subcutaneous tissue of mice was observed on the 7th, 14th and 28th days, and the results of hematoxylin-eosin staining, the collagen fibercontent and the number of capillaries and small blood vessels were analyzed in each group. Results Compared with other injection materials, the area injected with β-glucan combined with low cross-linked sodium hyaluronate had better histocompatibility, more significan collagen fiber and angiogenesis, and no muscle swelling and no adverse reactions at the injection site. There was significant differencein the collagen fibercontent in the slice images of rats in each group (P<0.05), among them, the collagen fibercontent in the β-glucan+low-crosslinking sodium hyaluronate group was the highest, followed by the cross-linking sodium hyaluronate+low-crosslinking sodium hyaluronate group, and the collagen fibercontent in the low-crosslinking sodium hyaluronate group, the cross-linking sodium hyaluronate group and the β-glucan group was lower than that in the firsttwo groups. The number of capillaries and small blood vessels in the cross-linked sodium hyaluronate group and the β-glucan+low cross-linked sodium hyaluronate group was higher than that in the low cross-linked sodium hyaluronate group, the β-glucan group and the cross-linked transparent sodium hyaluronate+low cross-linked sodium hyaluronate group, the differencewas statistically significant(P<0.05); there was no significantdifferece in the number of capillaries and small blood vessels between the cross-linked sodium hyaluronate group and the β-glucan+low cross-linked sodium hyaluronate group (P>0.05); there was no significantdifferencein the number of capillaries and small blood vessels between the low cross-linked sodium hyaluronate group and the β-glucan group and the cross-linked sodium hyaluronate+low cross-linked sodium hyaluronate group (P>0.05). Conclusion The subcutaneous injection of β-glucan combined with low cross-linked sodium hyaluronate has good histocompatibility. By comparing the area of collagen fibers,the number of capillaries and small blood vessels of differentcombination injection materials, it is better than other injection materials. Angiogenesis and new collagen have obvious and safe effects