Objective To investigate the method of chondrocyte proliferation in REBORN Wnt-5ARor2 signaling pathway. Methods The culture medium of chondrocytes was prepared to prepare and culture chondrocytes. The effects of different concentrations of cell proliferation preparations on the growth of chondrocytes were analyzed. At the same time, the effects of two proliferation preparations on the growth of chondrocytes were added. WESTERN was used to detect the signal pathways of aloe-emodin and caffeic acid on cell proliferation. Results The addition of aloe-emodin and caffeic acid at the corresponding concentration could promote the culture of chondrocytes, and its proliferation effect was better than other media. Conclusion REBORN can regulate IL-1β-induced chondrocyte proliferation and regeneration by inhibiting Wnt-5ARor2 signaling pathway