Objective To investigate the clinical effect of JUYOU medical recombinant type Ⅲ humanized collagen repair ointment combined with compound glycyrrhizin tablets and tacrolimus ointment in the treatment of facial hormone-dependent dermatitis and its effect on skin barrier function indexes. Methods A total of 78 patients with facial hormone-dependent dermatitis admitted to the Department of Dermatology of our hospital from March 2020 to May 2022 were selected as the research objects. They were divided into control group and observation group by random number table method, with 39 cases in each group. The control group was treated with compound glycyrrhizin tablets combined with tacrolimus ointment. The observation group was treated with JUYOU medical recombinant type Ⅲ humanized collagen repair ointment on the basis of the control group. The clinical efficacy, skin barrier function index, subjective and objective scores of facial symptoms and signs, quality of life and adverse reactions were compared between the two groups. Results The total effective rate of the observation group was 92.31%, which was higher than 71.79% of the control group (P<0.05). After treatment, the epidermal water loss in the observation group was lower than that in the control group, and the epidermal water content and epidermal oil content were higher than those in the control group (P<0.05). The scores of facial symptoms and facial signs in the observation group were lower than those in the control group (P<0.05). The scores of skin disease quality of life index in the observation group were lower than those in the control group at 7 and 14 days after treatment (P<0.05). The incidence of adverse reactions in the observation group was 2.56%, which was lower than 17.95% in the control group (P<0.05). Conclusion The clinical effect of JUYOU medical recombinant type Ⅲ humanized collagen repair ointment in the adjuvant treatment of facial hormone- dependent dermatitis is good, which can effectively reduce the facial symptoms and signs of patients, improve the physiological function of skin barrier, and improve the quality of life.