Objective To explore the application of standardized nursing intervention in patients with upper limb injury after flap transplantation. Methods A total of 96 patients with upper limb injury after flap transplantation admitted to the Eighth Ward of Orthopedics Department, North China Medical and Health Group Xingtai General Hospital from January 2019 to February 2020 were selected as the research objects. According to the random number table method, they were divided into group A and group B, with 48 cases in each group. Group A was given routine nursing, and group B was given standardized nursing intervention. The hospitalization time, pain degree, flap survival rate and nursing satisfaction were compared between the two groups. Results The hospitalization time of group B was shorter than that of group A, the difference was statistically significant (P