Objective To prepare a cream for damaged skin, and to explore its repair and soothing effect. Methods A 5 mg/ml transdermal fibronectin solution was prepared, and the transdermal fibronectin penetration test was performed using suckling pig skin through in vitro penetration test. The skin model was randomly divided into BC group, NC group, PC group and sample group. Collagen fiber staining and HE staining were performed in each group for lipid content analysis and observation based on human facial images. Results Fluorescence integral optical density (IOD) analysis of transdermal fibronectin showed that after 24 h of penetration, the penetration of transdermal fibronectin in tissues was significantly higher than that of fibronectin (P<0.01). After 2, 6 and 24 h of permeation, the permeation amount of transdermal fibronectin in the receiving solution was significantly hig(P<0.01). The integral optical density values of PC group and sample group were significantly higher than those of NC group (P<0.01). The results of FLG immunofluorescence showed that compared with BC group, the fluorescence signal of NC group was weakened, and the content of FLG was significantly decreased (P<0.01). Compared with the NC group, the FLG fluorescence signal of the PC group and the sample group increased significantly, and the FLG content increased significantly (P<0.01). Compared with NC group, the fluorescence signal of PC group and sample group was significantly enhanced (P<0.01). Compared with the BC group, the cholesterol content, fatty acid content and ceramide content in the PC group and the sample group were significantly increased (P<0.01), and the tissue morphology of the transdermal fibronectin group and the red area of the patient's face were significantly improved. Conclusion The permeability of transdermal fibronectin is significantly better than that of ordinary fibronectin, which can effectively improve the damage of collagen fibers in dermal skin model and promote dermal repair. Fibronectin facial cream can effectively improve the tissue morphology of EpiKutis? , a 3D epidermal skin model stimulated by SLS, significantly increase the content of silk polyprotein (FLG) and barrier lipids (cholesterol, ceramide, fatty acid), and improve the red area of facial skin.