脂肪干细胞外泌体抑制 TGF-β1 促进糖尿病创面愈合的研究
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宋培军(1988.10-),男,安徽临泉县人,博士,主治医师,主要从事皮瓣及创面修复方面研究

通讯作者:

徐静(1969.3-),男,安徽巢湖人,本科,教授,主任医师,主要从事皮瓣及创面修复方面研究

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R587.1

基金项目:

1.蚌埠医学院自然科学研究重点项目(编号:2021byzd044);2.2022年省级大学生创新创业训练计划项目(编号:12110110447)


Study on Adipose-derived Stem Cell Exosomes Inhibiting TGF-β1 to Promote Diabetic Wound Healing
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    摘要:

    目的 检测脂肪干细胞外泌体(ADSC-Exos)对糖尿病创面愈合、瘢痕形成的影响,并以 TGF-β1信号为靶点,探讨ADSC-Exos发挥作用的分子机制。方法 分离培养脂肪干细胞,收集外泌体进 行鉴定。并在糖尿病大鼠创面多点注射外泌体,通过计算创面面积、组织学染色以及Western Blot检测, 评估外泌体对创面愈合、TGF-β1信号通路的影响。结果 获取的ADSC-Exos符合典型的外泌体特征; ADSC-Exos可提高创面愈合率和血管化、抑制瘢痕,并抑制TGF-β1信号通路。结论 ADSC-Exos促进了 大鼠糖尿病创面愈合,在创伤后期可抑制关键纤维化信号TGF-β1,减少瘢痕形成

    Abstract:

    Objective To detect the effect of adipose-derived stem cell exosomes (ADSC-Exos) on diabetic wound healing and scar formation, and to explore the molecular mechanism of ADSC-Exos with TGF-β1 signal as the target. Methods Adipose-derived stem cells were isolated and cultured, and exosomes were collected for identification. Exosomes were injected into the wounds of diabetic rats, the effects of exosomes on wound healing and TGF-β1 signaling pathway were evaluated by calculating wound area, histological staining and Western Blot detection. Results The obtained ADSC-Exos conformed to the typical characteristics of exosomes; ADSC-Exos could improve wound healing rate and vascularization, inhibit scar formation, and inhibit TGF-β1 signaling pathway. Conclusion ADSC-Exos promotes wound healing in diabetic rats, inhibits the key fibrosis signal TGF-β1 and reduces scar formation in the later stage of trauma

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宋培军 ,梁 秋,等.脂肪干细胞外泌体抑制 TGF-β1 促进糖尿病创面愈合的研究[J].医学美学美容,2023,32(13):48-52.

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  • 在线发布日期: 2023-09-26
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