Objective To evaluate the effects of fluorine incorporation into porcine hydroxyapatite (PHA) on attachment, proliferation and osteogenic differentiation of rat pre-osteoblast cell line MC3T3-E1. Methods PHA was fabricated from porcine bone by thermal treatment. Fluorine incorporation was carried out by sodium fluoride immersion and a second stage thermal treatment. The fluorinated porcine hydroxyapatite (FPHA) bone blocks were prepared, grinded to obtain particle materials, and then prepared into tablets. The tablets prepared by PHA particles were used as the control group, and the tablets prepared by FPHA particles were used as the experimental group. Mouse MC3T3-E1 pre-osteoblast cell line was inoculated on the surface of the tableting material. The cell adhesion morphology at different times was observed by scanning electron microscopy (SEM). The cell proliferation was detected by CCK-8 method. The expression of osteogenic related genes alkaline phosphatase ALP, osteocalcin BGLAP and osteopontin OPN mRNA was detected by RT-PCR. Results SEM observation showed that the cells in the PHA and FPHA groups adhered and grew well. There was no significant difference in OD value between the two groups on 1st and 7th days after cell inoculation (P>0.05). The OD value of FPHA group was higher than that of PHA group on 3rd and 5th days after cell inoculation, and the difference was statistically significant (P＜0.05）ALP and BGLAP mRNA in FPHA group were higher than those in PHA group on 3rd and 7th days after cell inoculation, and the expression levels of ALP and BGLAP on the surface of the two groups on 7th day after cell inoculation were higher than those on 3rd day after cell inoculation, the differences were statistically significant (P<0.05). Conclusion PHA and FPHA materials have good cell compatibility. Fluorine incorporation into porcine hydroxyapatite can promote the early proliferation and osteogenic differentiation of mouse preosteoblasts.