廖阳阳（1984.7-），女，广东广州人，硕士，主治医师，主要从事牙周病科的临床工作
谢成婕（1979.4-），女，湖北宜昌人，博士，副主任医师，主要从事牙周病科的临床工作
R781
广东省医学科学技术研究基金-面上项目（编号：B2022024)
LIAO Yang-yang1, LI Si-bei2, ZHANG Cai-mei3, XIE Cheng-jie1
1.Department of Periodontal Disease, Haizhu Square Campus, Stomatological Hospital, Southern Medical University,2.The Third Department of Surgery, Guangzhou Chest Hospital,3.Department of Dentistry and Endodontics, Haizhu Square Campus, Stomatological Hospital, Southern Medical University,目的 研究浓缩生长因子（CGF）在炎症微环境下对人牙周膜细胞（hPDLCs）成骨抑制作用的 影响。 方法 分离、培养并鉴定hPDLCs，并采用脂多糖（LPS）制造细胞炎症微环境，分别将全血（对照 组）、Con组、LPS组和CGF组与hPDLCs共培养，使用1%、5%、10%的LPS、CGF分别对hPDLCs处理24、 48、72 h，进行成骨诱导，观察不同TGFβ1和IGF-1浓度下各组CGF中含有存在成骨相关生长因子水平、 炎症微环境下hPDLCs的各组ALP活性、qPCR检测各组成骨相关生长因表达量。 结果 CGF+DMEM培养 基中TGFβ1、IGF-1浓度均显著高于DMEM培养基、CGF析出液（P＜0.01）；hPDLCs细胞较为均一，无 明显杂质细胞，形态稳定，生长旺盛，进一步对hPDLCs进行LPS及CGF干预后，与Con组比较，ALP在LPS 组明显下降，而经过CGF干预后ALP的浓度有所回升，甚至比Con组更高；CGF组干预后ALP活性显著高 于对照组、LPS组，且LPS组低于对照组、炎症组（P＜0.01）；炎症微环境下，CGF组hPDLCs成骨标志物 ALP、COL1及OCN的表达量显著高于Con组、LPS组（P＜0.01）。 结论 CGF在炎症微环境下对改善人牙 周膜细胞成骨抑制作用具有积极的影响，可提高成骨相关因子（ALP、COL1、OCN）水平，促进成骨分 化的相关转录因子表达量，从而有效修复人牙周膜细胞在炎症微环境下的成骨抑制作用。
the inflammatory microenvironment, and the expression of bone-related growth factors in each group by qPCR. Results The concentrations of TGFβ1 and IGF-1 in CGF+DMEM medium were signifcantly higher than those in DMEM medium and CGF precipitation solution (P<0.01). The hPDLCs cells were relatively uniform, with no obvious impurity cells, stable morphology and vigorous growth. After further LPS and CGF intervention on hPDLCs, compared with the Con group, ALP decreased signifcantly in the LPS group, while the concentration of ALP increased after CGF intervention, even higher than the Con group; the ALP activity of the CGF group was signifcantly higher than that of the control group and the LPS group, and that in the LPS group was lower than that in the control group and the in?ammation group (P<0.01). Under the in?ammatory microenvironment, the expression levels of osteogenic markers ALP, COL1 and OCN in hPDLCs of CGF group were signifcantly higher than those of Con group and LPS group (P<0.01). Conclusion CGF has a positive e?ect on improving the osteogenic inhibition of human periodontal ligament cells in the in?ammatory microenvironment. Meanwhile, it can increase the levels of osteogenic related factors (ALP, COL1, OCN) and promote the expression of transcription factors related to osteogenic di?erentiation, so as to e?ectively repair the osteogenic inhibition of human periodontal ligament cells in the in?ammatory microenvironment.
廖阳阳,李思贝,张彩美,等. CGF在炎症微环境下对人牙周膜细胞成骨抑制作用的影响[J].医学美学美容,2023,32(18):65-69.
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