Objective To explore the clinical effect of microneedle radiofrequency combined with glycolic acid in the treatment of acne. Methods A total of 150 patients with acne admitted to our hospital from May 2021 to May 2022 were randomly divided into control group and study group, with 75 patients in each group. The control group was treated with glycolic acid peels, and the study group was treated with microneedle radiofrequency acne treatment instrument combined with glycolic acid peels. The clinical efficacy, ECCA score, nursing satisfaction and adverse reactions were compared between the two groups. Results The total effective rate of treatment in the study group was 97.33%, which was higher than 89.33% in the control group, and the difference was statistically significant (P<0.05). The ECCA score of the study group was lower than that of the control group, and the difference was statistically significant (P<0.05). The treatment satisfaction of the study group was higher than that of the control group, and the difference was statistically significant (P<0.05). There were no serious adverse reactions in both groups. Conclusion Microneedle radiofrequency acne treatment instrument combined with glycolic acid peels treatment has better effect on relieving and controlling the clinical symptoms of acne patients, with no obvious side effects, and the patients are more satisfied with the treatment effect, which is worthy of clinical application.