Objective To explore the application value of alginate impression materials of compound traditional chinese medicine disinfectant. Methods The alginate impression materials were prepared with the prepared individual trays. The impressions were immersed in the bacterial suspension of Staphylococcus aureus, Candida albicans, and Escherichia coli at a concentration of 108/ml, and then the infected alginate impressions were immersed in 0.25%, 0.50% Yirenan disinfectant and 2000 mg/L 84 disinfectant for 3, 5, 10, and 15 minutes, respectively. The sterilized impressions were immersed in 100 ml of 0.90% normal saline for 3 minutes and then removed. The live bacteria were cultured by pour culture method and counted. Results The sterilization rate of three disinfectants on Staphylococcus aureus, Candida albicans and Escherichia coli was 100.00% at 10 minutes and 15 minutes. At 3 minutes and 5 minutes, the sterilization rate of two concentrations of Yirenan disinfectant was 97.62%~98.61 %, and the sterilization rate of 84 disinfectant was 99.43%~99.64%. Conclusion The sterilization rate of Staphylococcus aureus, Candida albicans and Escherichia coli reached 100.00% when the alginate impression materials was soaked in 0.25% concentration of Yirenan disinfectant for more than 10 minutes.