Objective To evaluate the effect of poly-L-lactic acid combined with FemeTite on vaginal rejuvenation in women. Methods A total of 63 patients who underwent vaginal rejuvenation treatment in Hangzhou Jollyhue Yimei Medical Cosmetic Clinic from August to December 2023 were randomly divided into group A, group B and group C, with 21 patients in each group. Group A was treated with FemeTite, group B was treated with poly-L-lactic acid, and group C was treated with FemeTite+poly-L-lactic acid. The sexual function, biological electromyography index and vaginal local pH value of the three groups were compared. Results The FSFI score of the three groups at 1 and 2 months after treatment was higher than that before treatment, and the FSFI scores of the three groups at 1 and 2 months after treatment were significantly significant (P <0.05). The biological electromyography score of the three groups at 1 and 2 months after treatment was higher than that before treatment, and the biological electromyography scores of the three groups at 1 and 2 months after treatment were statistically significant (P <0.05). The vaginal pH value of the three groups at 2 months after treatment was higher than that before treatment, and the vaginal pH value of the the three groups at 2 months after treatment was statistically significant (P <0.05). Conclusion Poly-L-lactic acid combined with FemeTite can effectively improve female pelvic floor muscle function and vaginal pH value, so as to achieve vaginal rejuvenation.