Objective To analyze the effect of CO2 laser in the treatment of vaginal relaxation and its influence on vaginal mucosa related indexes. Methods From July 2021 to September 2022, 100 patients with vaginal relaxation treated in Xinjiekou Medical Cosmetology Clinic of Nanjing Jinying International Health Industry Group were selected as the research objects. According to different diagnosis and treatment methods, they were divided into observation group and control group, with 50 patients in each group. The control group was treated with modified posterior vaginal wall repair, and the observation group was treated with CO2 laser. The improvement effect of relaxation degree and vaginal mucosa related indexes were compared between the two groups. Results The improvement effect of relaxation in the observation group was better than that in the control group (P <0.05). The scores of vaginal mucosal elasticity, secretion type ( viscosity ), mucosal epithelial tissue and wettability in the observation group were higher than those in the control group (P <0.05). Conclusion CO2 laser treatment can effectively improve the degree of vaginal relaxation and improve the health status of vagina, which has high clinical application value.