Recombinant humanized collagen type Ⅲ is increasingly important in modern medicine due to its unique biocompatibility and biological activity. It can simulate the structure and function of natural type Ⅲ collagen in human body, which opens up a new direction for clinical treatment. The material has shown good application prospects and clinical effects in the fields of tissue engineering, skin repair, wound healing and plastic surgery. With the development of biotechnology, the development and application of recombinant humanized collagen type Ⅲ have been expanding, providing more choices for skin beauty. This article reviews the sources of recombinant humanized collagen type Ⅲ and its application in different fields, in order to provide reference for relevant researchers and clinical workers.