Objective: To explore the clinical efficacy of hyaluronic acid combined with type I collagen in the treatment of periorbital rejuvenation. Methods: From November 2024 to November 2025, 80 patients who underwent periorbital rejuvenation were included in the study. The subjects were divided into groups using the random number table method. 40 patients who received hyaluronic acid injection treatment were included in the control group, and 40 patients who received combined treatment with type I collagen were included in the study group. The differences in treatment effects between the two groups were evaluated. Results: The excellent rate of periorbital skin appearance in the study group was higher than that in the control group (P < 0.05); the levels of various indicators of skin barrier function in the study group after treatment were better than those in the control group (P < 0.05); the score of periorbital skin characteristics in the study group after treatment was lower than that in the control group (P < 0.05); the improvement rate of periorbital aging in the study group was higher than that in the control group (P < 0.05); the satisfaction of patients in the study group was higher than that in the control group (P < 0.05). Conclusion: Combined injection of hyaluronic acid and type I collagen in periorbital rejuvenation can synergistically improve the condition of periorbital skin and the degree of aging, enhance skin barrier function, and improve the overall effect and patient satisfaction of periorbital rejuvenation.