Objective To analyze the infection status of Demodex folliculorum (Df) and its risk factors in different subtypes of rosacea. Methods A total of 130 patients with rosacea diagnosed in the Department of Dermatology, the Second Afffliated Hospital of Xi'an Jiaotong University from June 2023 to June 2025 were selected as the research subjects, including 57 patients with erythematotelangiectatic rosacea (ETR), 53 patients with papulopustular rosacea (PPR), and 20 patients with phymatous rosacea (PHR). The Df infection status in patients with different subtypes of rosacea was statistically analyzed. According to the results of Df microscopic examination, the patients were divided into Df-positive group and Df-negative group. Univariate and multivariate Logistic analyses were performed to identify the independent risk factors for Df infection. Results The Df positive rate of 130 patients with rosacea was 64.62%, among which the Df positive rate of PPR patients was higher than that of other subtypes (P<0.05). There were statistically signiffcant differences in age, disease duration, facial skin type and sleep quality between Df-positive group and Df-negative group (P<0.05). Logistic regression analysis showed that PPR subtype, age >31 years, disease duration >3 years and oily skin were independent risk factors for Df infection in patients with rosacea (P<0.05). Conclusion Df infection is closely related to the occurrence and development of rosacea, and the distribution of Df infection varies among different subtypes, with a higher Df positive rate in PPR rosacea. In clinical practice, early screening and intervention for Df infection should be emphasized in high-risk populations, including patients with the PPR subtype, age >31 years, disease duration >3 years, and those with oily skin, which may help improve the therapeutic effect and prognosis.